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| An experimental study of curcumin inhibiting the malignant phenotype of renal cell carcinoma by regulating the TLR4/NF‑κB
signaling pathway |
| Lin Changwei, Yuan Xinzhu, Yuan Zujun |
| Department of Nephrology, Second Clinical College, North Sichuan Medical College, Nanchong 637001, Sichuan, China |
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Abstract Objective To explore curcumin inhibiting the malignant phenotype of human renal cell carcinoma (RCC) by
regulating the Toll⁃like receptor 4/nuclear factor⁃κB (TLR4/NF⁃κB) signaling pathway. Method The HK⁃2, 786⁃0, and ACHN
cell lines cultured in vitro were divided into control group, curcumin group, TLR4/NF⁃κB signaling pathway inhibitor group
(TAK⁃242 group). The curcumin group was divided into curcumin 10, 20, 40 μmol/L subgroup. CCK⁃8 method, Annexin
V⁃FITC/PI staining, scratch test, and Transwell chamber test were used to detect ability of cell proliferation, apoptosis, migration
and invasion; ELISA method was used to detect cell interleukin⁃1β (IL⁃1β), interleukin⁃6 (IL⁃6) and tumor necrosis factor⁃α (TNF⁃α) content; Western blotting method was used to detect the expression of TLR4, NF⁃κB p65, B cell lymphoma⁃2 (Bcl⁃2),
BCL2⁃Associated X (Bax), and matrix metalloprotein⁃9 (MMP⁃9). Result Curcumin had no toxic effect at concentrations below
40 μmol/L; in renal cancer cell lines 786⁃0 and ACHN, Compared with the control group, curcumin 10, 20, and 40 μmol/L
subgroups can significantly inhibit cell proliferation, migration and invasion, and promote cell apoptosis; IL⁃1β, IL⁃6 and TNF⁃α,
TLR4, NF⁃κB p65, Bcl⁃2, MMP⁃9 levels decrease, and Bax levels increase, The differences are statistically significant (P<0.05).
Conclusion Curcumin can inhibit the proliferation, migration and invasion of renal cancer cells and promote cell apoptosis by
inhibiting the TLR4/NF⁃κB signaling pathway.
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Received: 04 September 2021
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| Cite this article: |
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Lin Changwei,Yuan Xinzhu,Yuan Zujun. An experimental study of curcumin inhibiting the malignant phenotype of renal cell carcinoma by regulating the TLR4/NF‑κB
signaling pathway[J]. Electron J Metab Nutr Cancer, 2022, 9(1): 49-55.
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| URL: |
| http://182.92.200.144/EN/ OR http://182.92.200.144/EN/Y2022/V9/I1/49 |
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