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| Effect of down‑regulation of XRCC6 on colorectal cancer cells based on ATM/CHK2/p53 signaling pathway |
| 1 Liu Jiantong, 2 Fang Yu, 3 Gao Hongbo, 1 Song Mengmeng, 1 Lin Chao |
| 1
Department of General Surgery, Beijing Nuclear Industry Hospital, Beijing 102413,China; 2
Department of General Surgery, Xuanwu Hospital,
Capital Medical University, Beijing 100053, China; 3
Department of Radionuclide Treatment Center, Beijing Nuclear Industry Hospital, Beijing
102413, China |
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Abstract Objective To explore the effect of down⁃regulation of XRCC6 on apoptosis and invasion of colorectal cancer
cells and the changes of ATM/CHK2/p53 signaling pathway. Methods Human colorectal cancer SW620 cells were divided into
three groups: control group, si⁃NC group and si⁃XRCC6 group. MTT assay was used to detect cell viability. Flow cytometry was
used to detect cell apoptosis. Cell scratch test was used to detect cell migration. Transwell was used to detect cell invasion. qPCR
was used to detect ATM, CHK2 and p53 mRNA expression. Western blot was used to detect ATM, CHK2, p53 protein
expression and the phosphorylation level of p⁃ATM, p⁃CHK2 and p⁃p53. Results MTT results showed that the cell viability of
si⁃XRCC6 group was significantly lower than that of control group and si⁃NC group (P<0.05). Flow cytometry results showed
that the survival rate of si⁃XRCC6 group was lower than that of control group and si⁃NC group (P<0.01), the early apoptosis rate
was increased (P<0.01), the proportion of late apoptotic cells increased significantly (P<0.01), and there was no significant
difference between si⁃NC group and si⁃XRCC6 group (P>0.05).Compared with the control group and si⁃NC group, the migration
ability and invasion ability of si⁃XRCC6 group decreased (P<0.01). qPCR and Western blot results showed that the mRNA and
protein expression levels of ATM, CHK2 and p53 in si⁃XRCC6 cells increased, and the phosphorylation level of p⁃ATM,
p⁃CHK2 and p⁃p53 also increased. Conclusion Down regulation of XRCC6 can inhibit the activity of human colorectal cancer
cells and induce early apoptosis, which may be related to the regulation of ATM, CHK2 and p53 signaling pathways and their
phosphorylation levels.
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Received: 03 August 2021
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