1Department Ⅱ of Respiratory Sciences Affiliated Hospital of Hebei University of Engineering Handan 056000 Hebei China
2Department Ⅱof Oncology Affiliated Hospital of Hebei University of Engineering Handan 056000 Hebei China
Abstract Objective To explore the regulatory effect of lncRNA DRAIC on miR-223-3p and its effect on proliferation and metastasis of lung cancer cells. Method The expression of lncRNA DRAIC in lung cancer tissue and paracancer tissues and its relationship
with prognosis and survival time of 90 patients with non-small cell lung cancer were analyzed by TCGA data from January 2019 to June
2020 in Affiliated Hospital of Hebei University of Engineering. The expression of lncRNA DRAIC and miR-223-3p in lung cancer tissue was detected by real-time quantitative polymerase chain reaction RT-qPCR and the correlation of Pearson was analyzed. Starbase prediction and double luciferase test were used to verify the targeting relationship of lncRNA DRAIC to miR-223-3p. The lentivirus-packaged lncRNA DRAIC and miR-223-3p lentivirus plasmids were transfected into lung cancer cells A549 and H520 respectively and were divided into control group si-NC group si-DRAIC group pcDNA group pcDNA-DRAIC group DRAIC+miR-NC
group and DRAIC+miR-223-3p group. The ability of cell proliferation migration and invasion were detected by MTT assay and colony formation test scratch test and Transwell chamber test respectively. RT-qPCR and western blot method were used to detect the expression of mRNA and protein of JAK2 and STAT3. The tumorigenesis experiment in nude mice was used to detect the development of
transplanted tumors. Result TCGA data showed that the expression level of lncRNA DRAIC in lung cancer tissues was higher than that
in paracancer tissues and the prognostic survival time was negatively correlated with the expression level of lncRNA DRAIC. Compared with the paracancer tissues the level of lncRNA DRAIC in lung cancer tissues increased and the level of miR-223-3p decreased and the levels of lncRNA DRAIC and miR-223-3p showed a negative correlation. The level of lncRNA DRAIC is positively
correlated with tumor size TNM stage lymph node metastasis and differentiation P<0. 05 . There is a binding site between lncRNA
肿瘤代谢与营养电子杂志 2022 年 6 月 9 日第 9 卷第 3 期 Electron J Metab Nutr Cancer, Jun. 9, 2022, Vol. 9, No. 3 ·363·
DRAIC and miR-223-3p and the overexpression of lncRNA DRAIC inhibits the expression of miR-223-3p P<0. 05 and can upregulate the levels of mRNA and protein of JAK2 and STAT3 promote cell proliferation increase scratch healing and the number of invaded cells and promote the growth of transplanted tumors P<0. 05 . And miR-223-3p can partially reverse the malignant phenotype of lncRNA DRAIC on lung cancer cells. Conclusion lncRNA DRAIC promotes the proliferation migration and invasion of lung
cancer cells and the development of transplanted tumors in vivo by inhibiting miR-223-3p and it may play a role by activating the
JAK2 / STAT3 signaling pathway
1Wang Xiansheng,1Yu Hongyan,1Guo Xiaoyan, et al. lncRNA DRAIC promotes proliferation and metastasis of lung cancer cells by inhibiting miR-223-3p[J]. Electron J Metab Nutr Cancer, 2022, 9(3): 363-373.
