|
|
|
| Lung cancer cell (A549 cell) membrane phospholipid variation before and after radiotherapy |
| 1WU Wen-an, 2LI Chu-rong, 1FENG Po-ru, 1ZHAO Jin |
| 1WU Wen-an, 2LI Chu-rong, 1FENG Bo-ru, 1ZHAO Jin |
|
|
|
|
Abstract Objective To investigate the changes of A549 cell membrane phospholipids in lung cancer A549 cells before and after radiotherapy, and the relationship between cell membrane phospholipid metabolism and signal transduction pathway and the response of lung cancer cells to radiotherapy. Methods HPLC was used to detect the content of phospholipids in the lung cancer cells before and after 5Gy irradiation in 24 hours after A549 irradiation, to compare and analyse the content of phospholipids, in order to study the relationship between the change of the content and the effect of radiotherapy. Results The analytic result of HPLC showed the content of membrane phospholipid PI, PE and PC decreased in the A549 cell membrane after radiotherapy, with statistics significant deviation (P<0.01), PI: 1.53±0.07, 1.09±0.20, PE: 51.69±4.32, 32.47±6.60, PC: 128.79±7.40, 82.70±3.02, the content of membrane phospholipid PS is more in after radiotherapy than in before radiotherapy, but did not have statistics significant deviation. Conclusions The content of lipids decreased in lung cancer cell in radiotherapy. It is speculated that it is related to tumor cells, apoptosis and necrosis. Therefore, the study on the changes of lipids is of great significance in the treatment of lung cancer with stereotactic radiotherapy (SBRT).
|
|
|
|
|
|
| [1] |
Zheng Xianbin，Gao Jin，Hua Lei，Guo Weiqian2， Tao Zhenchao，Zhang Yangyang. Effect of depression on nutritional status of patients with nasopharyngeal carcinoma receiving radiotherapy[J]. Electronic Journal of Metabolism and Nutrition of, 2020, 7(2): 204-208. |
| [2] |
Chen Wei， Ding Qin，Chen Jian， Jiang Lei. Investigation on nutritional risk of patients with lung cancer surgery and its effect on clinical outcomes[J]. Electronic Journal of Metabolism and Nutrition of, 2020, 7(2): 214-219. |
| [3] |
Liu Caiping, Wei Lei, Liu Yanqing. Research progress on the relationship between vitamin B6 and lung cancer[J]. Electronic Journal of Metabolism and Nutrition of, 2020, 7(1): 112-116. |
| [4] |
1Wang Chunyan, 2Gu Wei, 2Hou Liping, 2Geng Jianlin, 2Zhang Xuekun. Effect of β-HB, LAC, Lp-PLA2 level monitoring on diabetic ketoacidosis combined infection[J]. Electronic Journal of Metabolism and Nutrition of, 2020, 7(1): 93-97. |
| [5] |
1Tang Minyu, 2Zeng Yuan, 2Hao Zhexue, 2Zhang Yalei. Vitamin and mineral supplements for the prevention and treatment of lung cancer[J]. Electronic Journal of Metabolism and Nutrition of, 2020, 7(1): 117-121. |
| [6] |
LI Hua-chun，JIANG Qing-hua，ZHAO Ji-li，YANG Rui，ZHANG Dan. Effect of standardized nutrition management on nutritional status of patients with head and neck cancer radiotherapy[J]. Electronic Journal of Metabolism and Nutrition of, 2019, 6(4): 468-471. |
|
|
|
|
