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| Screening and functional analysis of genes related to necrotizing apoptosis in patients with lower-grade glioma |
| 1Lyu Ying,2Yuan Pengrui,1Xu Jingxuan |
| 1Neurosurgery Department The Second Affiliated Hospital of Xinjiang Medical University Urumqi 830028 Xinjiang
China
2Orthopaedics The Second Affiliated Hospital of Xinjiang Medical University Urumqi 830028 Xinjiang China |
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Abstract Objective To determine the expression and prognostic significance of necrotic apoptosis-related genes in patients with
lower grade gliomas. Method The clinical practice data and sequencing data of lower grade gliomas were integrated from the TCGA
database and the necrosis-related genes retrieved from the database were compared and analyzed. First univariate Cox regression was
used to screen the genes of necrotic apoptosis related to the prognosis of patients with lower grade glioma and then multivariate Cox
regression was used to screen the genes that have the effect of regulating the prognosis. Through GO enrichment of
prognostication-related genes in high and low risk groups the factors that may affect the risk difference were summarized and then the
immune function status of high and low risk groups was investigated using ssGSEA analysis data. Result Seven genes were identified by
Lasso regression and lower grade glioma prognosis using univariate Cox regression and the high-risk and low-risk groups could be
distinguished by the prognostic risk scoring model of these seven genes. Kaplan-Meier data survival analysis showed that the overall
survival rate of the low-risk group was much higher than that of the high-risk group. The GO enrichment data analysis showed that
there was a significant difference in the immunity of the high - risk group compared with the low - risk group while the ssGSEA
enrichment analysis showed that the scores of most immune cells in the high - risk group were also significantly different from the
low-risk group and the scores of the low-risk group were lower. Conclusion The seven genes of FADD FAS MLKL RIPK1
RIPK3 TLR3 and TNF are significantly associated with necrotic apoptosis in patients with lower grade gliomas and can be used as the
next target for the treatment of lower grade gliomas.
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Received: 19 July 2022
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