Effect of miR-221-5p on biological behavior of gastric cancer cells by targeting regulation of ALDH1A2
1Yang Taoyong ，2Qian Kun ，1Zhu Dan ，1Jiang Xianxue
1Department of General Surgery Yuechi People ' s Hospital Yuechi District Guang ' an 638300 Sichuan China
2Department of
Gastrointestinal Surgery the First Affiliated Hospital of Chongqing Medical University Chongqing 400000 China
Abstract：Objective To investigate the targeting relationship between miR-221-5p and aldehyde dehydrogenase-1 family member
A2 ALDH1A2 and its effect on proliferation migration invasion and epithelial-mesenchymal transformation EMT of gastric cancer
cells. Method Gastric cancer tissues and their adjacent normal tissues were obtained from 50 patients with gastric cancer by radical
gastrectomy in Yuechi People's Hospital from July 2020 to July 2022. The cell lines cultured in vitro included human normal gastric
mucosal epithelial cell line GES-1 and human gastric cancer cell lines BGC-803 AGS SGC-7901 BGC-823 and HGC-27. The
expression of miR- 221- 5p and ALDH1A2 mRNA in tissues and cells was detected by qRT-PCR. The correlation between miR-
221-5p expression and clinicopathological parameters in gastric cancer was analyzed by Pearson test. The targeting regulation of
miR-221-5p on ALDH1A2 was verified by bioinformatics prediction and dual luciferase experiment. HGC-27 cells were divided into
control group inhibitor-NC group miR- 221 - 5p inhibitor group mimic NC group miR- 221 - 5p mimic group miR- 221 - 5p
mimic + pcDNA3. 1 group and miR - 221 - 5p mimic + pcDNA3. 1 - ALDH1A2 group and the corresponding plasmids were
transfected. Cell proliferation invasion migration apoptosis and cell cycle distribution were detected by MTT method clone
formation test Transwell chamber test scratch test and flow cytometry respectively. The expression of EMT-related proteins in cells
was analyzed by Western blot. Result The expression of miR-221-5p was significantly high in gastric cancer tissues and cells while
the expression of ALDH1A2 mRNA was significantly down-regulated in gastric cancer tissues P< 0. 05 . The expression of miR-
·69· 肿瘤代谢与营养电子杂志 2023 年 2 月 9 日第 10 卷第 1 期 Electron J Metab Nutr Cancer, Feb. 9, 2023, Vol. 10, No. 1
221-5p was significantly correlated with TNM stage tumor differentiation and lymph node metastasis in patients with gastric cancer
P<0. 05 . MiR-221-5p targeted inhibition of ALDH1A2 expression. Compared with anti-miR-NC group the OD value scratch
healing rate E-cadherin α-catenin protein expression and the proportion of S phase cells in anti-miR-221-5p group decreased
significantly while the expression level of E- cadherin and α - catenin protein and the proportion of G0 / G1 phase cells increased
significantly while the number of monoclonal formation and invasion decreased significantly P<0. 05 . The miR-221-5p group of
cells obtained the opposite results as described above when compared to the miR-NC group. Up-regulation of ALDH1A2 expression can
reverse the effect of miR-221-5p on the malignant biological behavior of HGC-27 cells. Conclusion Overexpression of miR-221-5p
may promote proliferation migration invasion EMT and cell cycle progression and inhibit apoptosis of gastric cancer cells through
targeted down-regulation of ALDH1A2 expression.
