miR-125b抑制胶质瘤细胞增殖作用机制研究

doi:10.16689/j.cnki.cn11-9349/r.2020.01.012

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摘要目的探讨 miR-125b抑制胶质瘤细胞增殖的分子作用机制。方法选取2016年12月至2017年12月宁夏医科大学总医院收治的胶质瘤患者32例（男18例、女14例），选择同期行脑内血肿清除术的脑出血或因颅脑损伤行颅内减压术的但脑组织正常患者31例为对照组（男15 例、女 16 例）。利用qPCR法检测miR-125b在胶质瘤组织和人胶质瘤细胞系 LN229的表达，通过转染miR-125b 模拟物，检测miR-125b对胶质瘤细胞LN229增殖的抑制作用，采用Targetscan、miRanda等预测软件对miR-125b的作用靶点进行预测，以双荧光素酶报告基因系统分析miR-125b与STAT3的相互作用，运用Western blot法检测miR-125b对胶质瘤细胞LN229中STAT3表达的影响。结果与正常癌旁脑组织相比较，胶质瘤组织中miR-125b的表达水平明显降低，胶质瘤细胞系LN229中miR-125b的表达与正常星形胶质细胞明显降低，相比于NC 模拟物，转染miR-125b 模拟物之后LN229细胞的增殖显著抑制，双荧光素酶报告基因系统检测显示，miR-125b 可直接调控STAT3 转录活性，而且miR-125b显著抑制LN229细胞STAT3表达。结论 miR-125b可靶向STAT3的表达从而调控胶质瘤细胞的增殖。

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	1陈鹏飞
	1霍显浩
	1刘文庆
	1李凯
	2薛涛

关键词 ：胶质瘤, 增殖, 信号传导与转录激活因子3, 作用机制

Abstract：Objective To explore the molecular mechanism of miR-125b inhibiting proliferation of glioma cells. Methods A total of 32 glioma patients were included in the research from December 2016 to December 2017, 31 patients who were with cerebral hemorrhage undergoing intracerebral hematoma removal or with intracranial decompression due to craniocerebral injury but normal brain tissuenormal were collected as the control. The expressions of microRNAs-125b in glioma tissue and LN229 cells were detected by qPCR. The inhibitory effect of microRNAs-125b on the growth of LN229 cells was detected after transfecting microRNAs-125b mimics. The target of microRNAs-125b was predicted by targetscan, miRanda and other prediction softwares. Dual luciferase reporter gene system was used to detect the interaction between RNA-125b and STAT3 mRNA, and the effect of RNA-125b on the expression of STAT3 protein in LN229 cells was detected by Western blot. Results Compared with normal para-cancerous brain tissues, the expression of microRNA-125b in glioma tissue was significantly decreased, and the expression of microRNA-125b in glioma cell line LN229 was also significantly lower than that in normal astrocytes. Compared with NC mimics, the proliferation of LN229 cells was significantly inhibited after transfected microRNA-125b mimics. Dual luciferase reporter gene system studies have shown that the transcriptional activity of STAT3 can be directly regulated by microRNAs-125b which also significantly inhibit the expression of STAT3 protein in LN229 cells. Conclusion The low expression of miR-125b in glioma cells is associated with poor prognosis. MiR-125b can regulate the proliferation of glioma cells by inhibiting the expression of STAT3.

Key words： Glioma Proliferation Signal transducer and activator of transcription Mechanism

通讯作者: 薛涛，电子邮箱：xuetao-80@163.com

引用本文:

1陈鹏飞，1霍显浩，1刘文庆，1李凯，2薛涛. miR-125b抑制胶质瘤细胞增殖作用机制研究[J]. 肿瘤代谢与营养电子杂志, 2020, 7(1): 64-69.
1Chen Pengfei,1Huo Xianhao,1Liu Wenqing,1Li Kai, 2Xue Tao. Mechanism of miR-125b inhibiting proliferation of glioma cells. Electron J Metab Nutr Cancer, 2020, 7(1): 64-69.

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