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| JC724 extraction and its effect on colorectal cancer |
| 1DENG Li, 2DING Yan, 1, 3RAO Ben-qiang, 1YANG Jun |
1Institute of Gastroenterology ,The Third Affilliated Hospital of Nanchang University, Nanchang 330006, China; 2Operation Room, 105 Hospital of the People’s Liberation Army, Hefei 230031, China; 3
Department of General Surgery/Cancer Medical Center,
Aviation General Hospital / Beijing Institute of Translational Medicine, Chinese Academy of Sciences, Beijing 100012, China |
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Abstract Objective To standardize the extraction condition of JC724 from natural plants, and to test the effect of JC724 on
colorectal cancer. Methods Standard operating procedure (SOP) was adopted to extract JC724 from preset medicine homologous
food and traditional anti-tumor Chinese medicine. Phytomics QC system was used to test stability of the extraction methods and to
examine the biological effect of JC724. By standardization of the extraction conditions and methods of JC724, the effect of inhibition
on colorectal cancer in vivo and in vitro was studied and compared with 5-Fu. Results 30 batches of JC724 were acquired using SOP
through ten different extraction conditions and methods (3 batches per set). Phytomics QC system was used to test for JC724. The
condition and method for the sample set which had the most stable biological activities was confirmed as standard extraction method.
During 96-hour, the highest inhibition rate on 293 cell line among JC724(20mg/ml), 5-Fu (5mg/ml), 5-Fu combined with JC724 (5-Fu:
2.5mg/ml, JC724: 10mg/ml) were 93.2%、82.1%、3.9% respectively, no effects was found in JC724 to 293 cell (P>0.05). The 96-
hour highest inhibition rate on HCT-116 among JC724, 5-Fu and 5-Fu+JC724 were 92.5%, 97.0% and 100% respectively (P>0.05).
However, the 96-hour inhibition rate on HT-29 among JC724, 5-Fu and 5-Fu+JC724 were 84.2%, 91.7% and 98.5% respectively
(P<0.05), and similar results were found in SW-480 cell line, the 96-hour inhibition rate on HT-29 among JC724, 5-Fu and 5-Fu+JC724
were 84.3%, 98.6% and 100% respectively (P<0.05). In colorectal tumor bearing mice model, the tumor inhibition rate of JC724, 5-Fu
and JC724+5-Fu were 83.95%, 85.60%and 92.07% respectively. After 9 days treatment, the weight of the control, JC724, 5-Fu and
JC724+5-Fu treated mice group were 106%, 104%, 73% and 86% of its primary weight respectively (P<0.01 among JC724, 5-Fu and
JC724+5-Fu group). Conclusions Phytomics QC system was a feasible evaluation tool for standardization of JC724, served as an
inhibitor on colorectal cancer in vivo and in vitro, which had synergistic effect with 5-Fu and might reduce the toxicity of 5-Fu.
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