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| Correlation between the expression and prognosis of unsaturated fatty acid-related enzymes CBR1 ALOX15 and FADS1 in
epithelial ovarian cancer |
| 1Lei Yuchen,1Li Ting,1Wang Rixin,2Lyu Haixu,1Jin Xiaoming,3Kong Dan,1Liu Yu |
| 1Department of Pathology School of Basic Medicine Harbin Medical University Harbin 150081 Heilongjiang China
2 No. 4 General
surgery department Heilongjiang Provincial Hospital Harbin 150036 Heilongjiang China
3Department of Gynecology Affiliated
Cancer Hospital of Harbin Medical University Harbin 150040 Heilongjiang China |
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Abstract Objective Exploring the correlation between the expression and prognosis of monounsaturated fatty acid related enzyme
CBR1 polyunsaturated fatty acid related enzymes ALOX15 and FADS1 in epithelial cancer tissues. Method The expression levels of
CBR1 ALOX15 and FADS1 were analyzed by immunohistochemistry and Western blot analysis from 65 epithelial ovarian cancer
samples collected from the Department of Gynecology Affiliated Cancer Hospital of Harbin Medical University from April 2020 to
December 2023. Analyze the expression of these three unsaturated fatty acid-related enzymes tumor cell proliferation correlation
with overall patient survival and immune cell infiltration through GEPIA2 database Kaplan-Meier survival curve and TIMER2. 0
database. Result Study found that CBR1 was correlated with serum CA125 levels ALOX15 was correlated with epithelial ovarian
cancer staging and P53 expression and FADS1 was correlated with differentiation degree all P<0. 05 . GEPIA2 database showed
that FADS1 was statistically significant with tumor stage and CBR1 and FADS1 were positively correlated with tumor cell proliferation.
Kaplan-Meier survival analysis showed that high expression of FADS1 predicted shorter survival while high expression of ALOX15
predicted longer survival. Western blot analysis of the expressions of three UFA-related enzymes in EOC tumor tissues and adjacent
tissues showed that the expressions of CBR1 and ALOX15 were consistent with GEPIA2 database while the expressions of FADS1 were
opposite. The expression of these three unsaturated fatty acid - related enzymes was correlated with immune cell infiltration
macrophages and CD8
+
T cells . Conclusion This study validated the correlation between the expression of CBR1 ALOX15 and
FADS1 in epithelial ovarian cancer and the clinical prognosis of patients However the relationship between monounsaturated fatty
acids or polyunsaturated fatty acids and tumor occurrence development and prognosis still requires a large amount of experimental
data for verification.
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| Cite this article: |
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1Lei Yuchen,1Li Ting,1Wang Rixin, et al. Correlation between the expression and prognosis of unsaturated fatty acid-related enzymes CBR1 ALOX15 and FADS1 in
epithelial ovarian cancer[J]. Electron J Metab Nutr Cancer, 2024, 11(6): 797-804.
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| URL: |
| http://www.ejmnc.com//EN/ OR http://www.ejmnc.com//EN/Y2024/V11/I6/797 |
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