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| Effects of vitamin C and heat treatment on the proliferation of lung cancer A549 cells via PI3K/ AKT/ HIF-1α pathway |
| Du Yanping, Ou Junwen, Liu Manting, Lu Zhitong, Wu Xiaofeng |
| Department of Nutrition Clifford Hospital Jinan University Guangzhou 511496 Guangzhou China |
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Abstract Objective To investigate the effects of vitamin C and heat treatment on lung cancer A549 cells and the related
mechanisms. Method Different concentrations of vitamin C The final concentration is 0 1 2 4 8 mmol / L 0 1 2 4 8 mmol / L
and different temperatures 37 ℃ and 41 ℃ were applied to A549 cells. CCK8 assay was used to detect the cell activity and FRASC
Vitamin C assay Kit II was used to detect the concentration of vitamin C in the cells. Then A549 cells were divided into control group
37 ℃ 0 mmol / L vitamin C vitamin C group 37 ℃ 8 mmol / L vitamin C heat treatment group 41 ℃ 0 mmol / L vitamin C
and combined group 41 ℃ 8 mmol / L vitamin C after 24 hours of intervention 2-NBDG was used to detect glucose uptake of A549
cells. The expression levels of GLUT1 GLUT3 PI3K AKT p-PI3K p-AKT and HIF-1α were detected by Western Blot assay.
The results were statistically analyzed by ANOVA and LST-t test. Result The cell viability of A549 decreased with the increase of
vitamin C intervention concentration P<0. 05 and the cell viability of 41 ℃ heat treated cells was lower than 37 ℃ after vitamin C
intervention. P < 0. 05 . The intracellular vitamin C level increased with the increase of intervention concentration and the
intracellular vitamin C level of 41 ℃ heat treated cells was lower than 37 ℃ after 2 4 and 8 mmol / L vitamin C intervention. P<
0. 05 . After the intervention compared to the control group the intracellular glucose intake of vitamin C group heat treatment group
and combined group was decreased P<0. 05 and the heat treatment group was lower than the vitamin C group P<0. 05 and the
combined group was lower than the vitamin C group and heat treatment group P < 0. 05 . Western Blot results showed that the
expression levels of GLUT1 PI3K P-PI3K p-AKT in the three intervention groups and GLUT3 HIF-1α in the vitamin C group,and GLUT3 HIF-1α and AKT in the combined group were lower than those in control group P<0. 05 . The protein expression levels
of GLUT1 PI3K p-PI3K in the combined group were lower than those in the vitamin C group P< 0. 05 and GLUT3 AKT
P-PI3K and HIF-1α in the combined group were lower than those in the vitamin C group and the heat treatment group P<0. 05 .
Conclusion Compared with vitamin C or 41 ℃ heat treatment vitamin C combined heat treatment significantly inhibited the PI3K/
AKT/ HIF-1α pathway of A549 cells down-regulated GLUT1 and GLUT3 reduced intracellular glucose uptake and inhibited A549
cell proliferations.
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