Study on the expression of CK2α in thyroid tumors and its mechanism of action in cancer metastasis
1 Dou Juan, 1 Li Hengyu, 1 Chen Jing, 2 Liu Zhou
1Department of Throat and Breast Surgery, the First Affiliated Hospital of Naval Military Medical University, Shanghai 200433, China;
2Central Plains Clinic, Dental Clinic, Yangpu District, Shanghai 200438, China
Abstract：Objective To investigate the expression level of CK2α in thyroid carcinoma, and to evaluate the biological
function and its mechanism of CK2α in thyroid carcinoma. Methods Twenty pairs of clinical thyroid carcinoma tissues and
corresponding paracancerous normal tissues were obtained, and the expression of CK2α in thyroid carcinoma tissues and
different human thyroid cancer cell lines were detected by qRT⁃PCR analysis. The effect of CK2α on cell proliferation of thyroid
cancer cells was detected by CKK⁃8 method. The effects of CK2α on the migration and invasion of thyroid cancer cells were
detected by wound healling test and Transwell test. The qRT⁃PCR was used to analyze the relationship between CK2α and
YBX1 expression. Results CK2α was significantly up⁃regulated in thyroid carcinoma tissues and cell lines. In addition,
decreased CK2α expression significantly inhibited the proliferation, migration and invasion of thyroid cancer cells, whereas
overexpression of CK2α promoted the proliferation, migration and invasion of thyroid cancer cells. Mechanistically, we found
that down⁃regulation of CK2α expression inhibited YBX1 expression, thereby inhibiting the biological function of thyroid cancer
cells. Conclusion CK2α promotes the development of thyroid cancer by promoting the expression of Y-box binding protein 1.
收稿日期: 2021-01-20
基金资助:上海市科研计划项目（18ZR1438600)
通讯作者:
李恒宇，电子邮箱：zhangdong8655@163.com
引用本文:
1窦娟，1李恒宇，1陈静，2刘宙. CK2α 在甲状腺肿瘤中的表达及其在癌转移中的作用
机制研究[J]. 肿瘤代谢与营养电子杂志, 2021, 8(3): 261-266.
1 Dou Juan, 1 Li Hengyu, 1 Chen Jing, 2 Liu Zhou. Study on the expression of CK2α in thyroid tumors and its mechanism of action in cancer metastasis. Electron J Metab Nutr Cancer, 2021, 8(3): 261-266.
