不饱和脂肪酸相关酶 CBR1、ALOX15 和 FADS1 在上皮性卵巢癌组织中的表达与预后相关性分析

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摘要目的探讨单不饱和脂肪酸相关酶 CBR1 及多不饱和脂肪酸相关酶 ALOX15 和 FADS1 在上皮性卵巢癌(EOC)组织中的表达与预后相关性。方法选取 2020 年 4 月至 2023 年 12 月来自哈尔滨医科大学附属肿瘤医院妇科 65 例上皮性卵巢癌样本,进行免疫组化和 Western blot 分析 CBR1、ALOX15 和 FADS1 的表达水平。通过 GEPIA2 数据库、Kaplan-Meier 生存曲线、TIMER2. 0 数据库分析这 3 种不饱和脂肪酸相关酶的表达、肿瘤细胞增殖、与患者整体生存期的相关性,以及免疫细胞浸润的关系。结果 65 例免疫组化分析发现 CBR1 与血清中 CA125 值,ALOX15 与 EOC 分期、P53 表达,FADS1 与分化程度相关(均 P<0. 05)。 GEPIA2 数据库显示 FADS1 与肿瘤分期有统计学意义,CBR1、FADS1 与肿瘤细胞增殖呈正相关。 Kaplan-Meier 生存分析 FADS1 高表达预示患者较短的生存期,而高表达 ALOX15 预示着更长的生存期。 Western blot 分析 3 种 UFA 相关酶在 EOC 肿瘤组织和癌旁组织中的表达,CBR1、ALOX15 表达与 GEPIA2 数据库显示一致,而 FADS1 相反。这 3 种不饱和脂肪酸相关酶的表达与免疫细胞浸润(巨噬细胞和 CD8 + T 细胞)有一定的相关性。结论本研究验证了 CBR1、ALOX15 和 FADS1 在上皮性卵巢癌中的表达与患者的临床预后相关;但单不饱和脂肪酸或多不饱和脂肪酸与肿瘤发生发展及预后的关系,仍需大量的实验数据加以验证。

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	1雷雨晨
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	1刘宇

关键词 ：上皮性卵巢癌, 腹水, 不饱和脂肪酸, 单不饱和脂肪酸, 多不饱和脂肪酸, 耐药, 铁死亡, 预后

Abstract：Objective Exploring the correlation between the expression and prognosis of monounsaturated fatty acid related enzyme CBR1 polyunsaturated fatty acid related enzymes ALOX15 and FADS1 in epithelial cancer tissues. Method The expression levels of CBR1 ALOX15 and FADS1 were analyzed by immunohistochemistry and Western blot analysis from 65 epithelial ovarian cancer samples collected from the Department of Gynecology Affiliated Cancer Hospital of Harbin Medical University from April 2020 to December 2023. Analyze the expression of these three unsaturated fatty acid-related enzymes tumor cell proliferation correlation with overall patient survival and immune cell infiltration through GEPIA2 database Kaplan-Meier survival curve and TIMER2. 0 database. Result Study found that CBR1 was correlated with serum CA125 levels ALOX15 was correlated with epithelial ovarian cancer staging and P53 expression and FADS1 was correlated with differentiation degree all P<0. 05 . GEPIA2 database showed that FADS1 was statistically significant with tumor stage and CBR1 and FADS1 were positively correlated with tumor cell proliferation. Kaplan-Meier survival analysis showed that high expression of FADS1 predicted shorter survival while high expression of ALOX15 predicted longer survival. Western blot analysis of the expressions of three UFA-related enzymes in EOC tumor tissues and adjacent tissues showed that the expressions of CBR1 and ALOX15 were consistent with GEPIA2 database while the expressions of FADS1 were opposite. The expression of these three unsaturated fatty acid - related enzymes was correlated with immune cell infiltration macrophages and CD8 + T cells . Conclusion This study validated the correlation between the expression of CBR1 ALOX15 and FADS1 in epithelial ovarian cancer and the clinical prognosis of patients However the relationship between monounsaturated fatty acids or polyunsaturated fatty acids and tumor occurrence development and prognosis still requires a large amount of experimental data for verification.

Key words： Epithelial ovarian cancer Ascites Unsaturated fatty acid Monounsaturated fatty acids Polyunsaturated fatty acids Drug resistant Ferroptosis Prognosis

基金资助:国家自然科学基金(82103217)

通讯作者: 刘宇,电子邮箱:liuyu@ hrbmu. edu. cn

引用本文:

1雷雨晨,1李婷,1王日昕,2吕海旭,1金晓明,3孔丹,1刘宇. 不饱和脂肪酸相关酶 CBR1、ALOX15 和 FADS1 在上皮性卵巢癌组织中的表达与预后相关性分析[J]. 肿瘤代谢与营养电子杂志, 2024, 11(6): 797-804.
1Lei Yuchen,1Li Ting,1Wang Rixin,2Lyu Haixu,1Jin Xiaoming,3Kong Dan,1Liu Yu. Correlation between the expression and prognosis of unsaturated fatty acid-related enzymes CBR1 ALOX15 and FADS1 in epithelial ovarian cancer. Electron J Metab Nutr Cancer, 2024, 11(6): 797-804.