Study on the relationship between phenotype of tumor-infiltrating lymphocytes and clinicopathological characteristics and gut
microbiota in patients with colon cancer
Huang Chao, Fang Xinggang, Chen Lu
Department of Integrated Traditional Chinese and Western Medicine Taihe Hospital Affiliated Hospital of Hubei University of Medicine
Shiyan 442000 Hubei China
Abstract：Objective To explore the relationship between the phenotype of tumor - infiltrating lymphocytes TIL and
clinicopathological characteristics and gut microbiota in patients with colon cancer. Method From June 2017 to February 2023 76
colon cancer patients admitted to Taihe Hospital were collected as the colon cancer group and 76 healthy individuals who underwent
physical examinations during the same period were collected as the control group. The TIL phenotype expression levels of two groups
were detected fluorescent quantitative PCR was applied to detect gut microbiota and the clinical and pathological data of colon cancer
patients were collected. Spearman was applied to analyze the correlation between TIL phenotype and gut microbiota. Result Compared
with the control group the level of CD4
+
T cell in the colon cancer group was obviously increased 28. 05± 5. 48 % vs 32. 49±
6. 13 % while the levels of CD3
+
T cell and CD8
+
T cell were obviously reduced 60. 47 ± 6. 53 % vs 53. 17 ± 6. 37 %
30. 69±6. 38 % vs 24. 85 ± 6. 42 % P < 0. 05 . According to the median values of CD3
+
T cell 52. 62% CD8
+
T cell
25. 87% and CD4
+
T cell 31. 94% colon cancer patients were divided into 38 cases with high expression of CD3
+
T cell 38 cases
with low expression of CD3
+
T cell 38 cases with high expression of CD8
+
T cell 38 cases with low expression of CD8
+
T cell 38 cases with high expression of CD4
+
T cell and 38 cases with low expression of CD4
+
T cell. There were lymph node metastasis the
higher the degree of tumor differentiation the higher the TNM stage and the difference between high and low expression of CD3
+
T
cell CD8
+
T cell and CD4
+
T cell was statistically significant
χ
2 = 5. 330 4. 659 6. 786
χ
2 = 7. 962 10. 483 4. 343
χ
2 = 11. 120
7. 280 13. 300 P<0. 05 . Compared with the control group the numbers of Bacteroides fragilis Escherichia coli and Enterococcus in
the colon cancer group increased obviously 6. 58 ± 0. 83 lgN/ g vs 8. 59 ± 1. 36 lgN/ g 7. 36 ± 1. 04 lgN/ g vs 9. 73 ±
1. 25 lgN/ g 7. 21±1. 08 lgN/ g vs 9. 28±1. 74 lgN/ g while the numbers of Lactobacillus and Bifidobacterium decreased
obviously 8. 85±1. 46 lgN/ g vs 6. 34± 1. 22 lgN/ g 8. 94± 1. 09 lgN/ g vs 6. 17± 1. 32 lgN/ g P< 0. 05 . Spearman
correlation analysis showed that Bacteroides fragilis Escherichia coli and Enterococcus were negatively correlated with the proportion of
CD3
+
T cell and CD8
+
T cell in colon cancer patients r = - 0. 482 - 0. 459 - 0. 532 r = - 0. 438 - 0. 472 - 0. 508 P < 0. 05
Lactobacillus and Bifidobacterium were positively correlated with the proportion of CD3
+
T cell and CD8
+
T cell r = 0. 581 0. 507 r =
0. 536 0. 467 P<0. 05 Bacteroides flimsii Escherichia coli and Enterococcus were positively correlated with the proportion of CD4
+
T cell r = 0. 483 0. 495 0. 461 P<0. 05 and Lactobacillus and Bifidobacterium were negatively correlated with the proportion of
CD4
+
T cell r = -0. 563 -0. 579 P<0. 05 . Conclusion The phenotypes of CD3
+
T cell CD8
+
T cell and CD4
+
T cell in TIL of
colon cancer patients have certain relationship to the clinicopathological characteristics and gut microbiota.
黄 超,方兴刚,陈 璐. 结肠癌患者肿瘤浸润淋巴细胞表型与临床病理特征
和肠道菌群的关系研究[J]. 肿瘤代谢与营养电子杂志, 2024, 11(4): 547-552.
Huang Chao, Fang Xinggang, Chen Lu. Study on the relationship between phenotype of tumor-infiltrating lymphocytes and clinicopathological characteristics and gut
microbiota in patients with colon cancer. Electron J Metab Nutr Cancer, 2024, 11(4): 547-552.
